Coding

Part:BBa_K1349008:Design

Designed by: Alexia Satouf, Clara Bouyx, Aimeric Agaoua, Lambert Antoni, Vincent Castel   Group: iGEM14_Aix-Marseille   (2014-10-06)


sh3pep-lza


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 163
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 91


Design Notes

This part forms the third component of the synthetic protein-protein interaction system developped by Whitaker et al. It is designed to form the bridge between the leucine zipper module in part BBa_K1349007 and the SH3 domain in part BBa_K1349005.

Expression of this construct is expected to lead to interaction between proteins containing an SH3 domain (part BBa_K1349005) and those containing the complementary leucine zipper peptide (such as part BBa_K1349007).

Source

Amplified from pWW2181,Whitaker et al. PNAS 2012

References

Whitaker WR, Davis SA, Arkin AP, Dueber JE. 2012. Engineering robust control of two-component system phosphotransfer using modular scaffolds. Proc Natl Acad Sci U S A. 109(44):18090-5. doi: 10.1073/pnas.1209230109.